thymus vulgaris fiche technique
Aliquots were analyzed to determine secreted cytokines. Both IL-1 and IL-6 enhance the inflammatory process. Trouvé à l'intérieur â Page 46Appl. Microbiol., 25: 135â7. mann cm and markham jl (1998) A new method for determining the minimum inhibition ... comig (1999) Antimicrobial activity of the essential oils of Thymus vulgaris L. measured using a bioimpedometric method, ... From Iran. Anti-inflammatory effect of thymol has been reported on human neutrophiles incubated with 10 or 20 μg/mL of this compound [14]. Plant Archives Vol. Composition of the supercritical thyme extracts was carried out by a GC-2010 (Shimadzu, Japan), equipped with a split/splitless injector, electronic pressure control, AOC-20i autoinjector, GCMS-QP2010 Plus mass spectrometer detector, and a GCMS Solution software. Positivating, anti-microbial, anti-infectious, anti-bacterial, antiseptic, fungicide, viricide, vermifuge, tonic, neurotonic, utero-tonic, aphrodisiac, mild antispasmodic, Candidiasic stomatitis+++, flatulence, gastritis, enterocolitis, parasitic colitis, candidiasic cystitis, staphylococcal pyelonephritis, renal tuberculosis, viral prostatitis, candidiasic vaginitis, staphylococcal metritis and salpingitis, bronchitis, bronchopneumonia, pleuritis, tuberculoses, nerve fatigue++, muscular rheumatisms, psoriasis, warts, eczema, furuncle, abscess, herpes. Similar results have been described for essential oils extracted from Cinnamomum osmophloeum, a herb traditionally used in Asia as food and as a medicine, which contains cinnamaldehyde. Viable cells were plated at a density of cells/mL in 24 wells plates (100 μL and 1 mL, resp.) Supernatants were centrifuged at 12,000 rpm to remove debris and then stored at −80°C until cytokine analysis. Materials and methods Staphylococcus aureus, Bacillus subtilis, Escherichia coli, Candida albicans and Aspergillus niger were used for antimicrobial activity assay with using disc diffusion technique. Fleurs roses. Mes 12 huiles essentielles propose la trousse des 12 HE indispensables pour toute la famille et tous les besoins ! Bronchitis is an acute or chronic inflammation of the mucous lining of the bronchial tubes, the main airways carrying air from the trachea to the lungs. 2, 2019 pp. 19 No. The yield of TEO was 1.76% v/w. Main compounds presented in the supercritical extracts of the three thyme varieties were 1,8 cineole, thymol, camphor, borneol, and carvacrol. The pellet was washed with 200 mL of 75% Ethanol in DEPC-treated H2O and centrifuged at 7600, 4°C for 5 min. Well known from amateurs as well as chefs, this herb holds an important place in Mediterranean cuisine. Cardiovascular disease, the leading cause of death in Western countries, is a preeminent health problem worldwide [1]. 2018 Sep;32(9):1688-1706. doi: 10.1002/ptr.6109. Changes on production were dose dependentent and according to the thymol content of each species (Table 1). In the same way, the inhibitory activity of 1,8-cineol (eucalyptol) on cytokine production in cultured human lymphocytes and monocytes has been described [38]: particularly, in monocytes, inhibition of TNF-α, IL-1β, IL-6, and IL-8 was 99, 84, 76, and 65%, respectively, when monocytes were treated with 0.15 μg/mL of 1,8-cineol. Supercritical fluid extraction (SFE) is considered an attractive extraction method when compared to conventional techniques such as steam distillation or Soxhlet extraction because it avoids solute contamination with solvent residues and the degradation of thermolabile compounds [25]. These ox-LDL-treated cells showed an increase in total protein secreted (data not shown). Voltammetric Determination of Isopropylmethylphenols in Herbal Spices. : Mar 2013 PA-254009.08 - 1 - BD Hektoen Enteric Agar (HE Agar) INTENDED USE BD Hektoen Enteric Agar is a moderately selective and differential medium for the isolation and cultivation of gram-negative enteric micro-organisms, especially for the isolation of Shigella and Salmonella species from fecal specimens. 2020 Jan 23;25(3):502. doi: 10.3390/molecules25030502. The absorbance of each well was then read at 540 nm using a microplate reader (Sunrise Remote, Tecan). Trouvé à l'intérieur â Page 16UtvrÄeno je da glavni dio fenolnog sadržaja u selekcioniranoj drogi Thymus vulgaris pripada timolu , a tek mali dio karvakrolu . U selekcioniranoj drogi Thymus serpyllum taj je odnos obrnut . Ova konstatacija koju bi trebalo potvrditi ... 2.2. PMC Afterwards, the wells were washed with PBS and the treatment initiated. is a medicinal and culinary herb from the Southern European region known for its anti-infective, cardioprotective, gastroprotective, anti-inflammatory, and immunomodulatory activities since the Egyptian era. Un gramme contient environ 4000 graines. Bethesda, MD 20894, Help Miranda-Cadena K, Dias M, Costa-Barbosa A, Collins T, Marcos-Arias C, Eraso E, Pais C, Quindós G, Sampaio P. Antimicrob Agents Chemother. Copyright © 2012 A. Ocaña and G. Reglero. Connectez vous à votre compte. SUMMARY . Cherallier A. London:: Dorling Kindersley Limited; 1996. THP-1 cells were plated in 96-well plates, differentiated and incubated with different concentrations of extract for 24 and 48 hours at 37°C in 5% CO2. On the other hand, Thymus species (Thymus vulgaris, Thymus capitatus L., and Thymus decassatus Benth.) Regarding Thymus hyemalis, at 24-hour treatments, both fractions of this species induced similar cell responses. Objective Evaluated essential oil constituents and antimicrobial activity of Thymus daenensis compared with Thymus vulgaris. Fractionation conditions were as follows: separator 1 was kept at constant pressure and temperature of 15 MPa and 40°C, respectively, whereas separator 2 was maintained at a pressure of 2 MPa and a temperature of 40°C. Our results are concordant with Sharma et al. Thyme (Thymus vulgaris), an herb in the mint family, is often used as a spice in cooking. NCI CPTC Antibody Characterization Program. The model was constructed using 30 samples of authenticated Thymus vulgaris and challenged with 20 samples of different botanical origins. Trouvé à l'intérieur â Page 80Terminologie : Noms latins : - Thymus vulgaris L. Noms français : - Thym commun , Thym vulgaire , Farigoule pour les ... Mis à part le thym vulgaire , décrit dans cette fiche , la plus répandue et la plus intéressante du point de vue ... The plant and Extraction: Thymus vulgaris L. (labiatae) was collected from areas between Mousl and Dhook district and nearest Karkook City.. Rustaiyan A, Lajevardi T, Rabbani M, Yari M, Masoudi Sh. To determinate whether thyme oils could have immunomodulation properties and could mediate in inflammatory cytokines regulation, we study the effect of our extracts on ox-LDL-activated THP-1 macrophages, measuring the expression and release of several inflammatory mediators. IL-10 expression gene increased twice with all thyme extracts at 24 hours of treatment compared with oxLDL-activated cells. Macrophages and foam cells produce inflammatory mediators responsible for the lesion growth. TNFα gene expression decreases only in cells treated with Thymus zygis S1 at 24 hours of incubation; other treatments did not change expression of this gene with respect to oxLDL-activated cells. Trouvé à l'intérieur â Page 117For basophile holm oaks , the following species were defined : Quercus coccifera , Thymus vulgaris , Genista scorpio , Rhamnus lycioides , Lithospermum fruticosum , Rosmarinus officinalis , Thymus zygis , Cistus albidus , and Salvia ... How do you revive thyme that has died? Thyme (Thymus vulgaris) Oregano (Origanum compactum) Wintergreen (Gaultheria procumbens) Cypress (Cupressus sempervirens) Peppermint (Mentha piperita) Basil (Ocimum basilicum) Marjoram (Origanum majorana) The oils are dispensed in little drops from a height of about six inches above the back, which is where Raindrop Technique gets its name. The genus Thymus comprises about 300 species of perennial aromatic, herbaceous plants with many subspecies, varieties, subvarieties and forms. Regarding IL-1β release at 24 hours, treatment of activated cells with any of the thyme fractions induced a decrease of this cytokine secretion in a dose-dependent manner (Figure 3(b)). Others have demonstrated to have antiatherogenic properties such as IL-10 that involve in the attenuation of the monocyte differentiation into macrophages [3]. The Egyptians and Etruscans would use thyme and other plants to preserve their dead. Accessibility Evaluation of the Activity of Crude Alkaloids Extracts of Zingiber officinale Roscoe., Thymus vulgaris L. and Acacia arabica L. as Coagulant Agent in Lab Mice. [39]. Cytokines exhibit both beneficial and pathologic effects on their target cells and are produced by many cell types [32]. Also, thymol and carvacrol prevent autoxidation of lipids [37]. Total RNA was then dissolved in 15 μL of DEPC H2O, incubated at 55°C for 10 minutes, and stored at −80°C for future use. This process becomes macrophage into foam cells that characterize the early atherosclerotic lesion [4]. 2015 Jan-Feb;26(1):61-71. doi: 10.1002/pca.2537. The use of medicinal plants, or extracts from them, has been traditionally practiced worldwide in the prevention and treatment of several chronic diseases such as cardiovascular diseases, intestinal inflammatory diseases, inflammatory bowel disease, arthritis, diabetes, allergies, multiple sclerosis, Parkinson’s and Alzheimer’s diseases, and others [5]. The obtained essential oil was dried over sodium sulphate and stored at 4 C in dark vials until tested. It is a bushy, woody based shrub, 10 to 40 cm high with small and highly aromatic gray-green oval leaves containing numerous small glands with clusters of pink or purple flowers. 2 College of Pharmacy, Al-Mustansyria University, baghdad, Iraq Trouvé à l'intérieur â Page 114Rather than a theoretical approach to this subject, we will use a bioactive natural product to highlight the strengths of these techniques. In a project to isolate and characterize antibiotics from plants, Thymus vulgaris (thyme), ... human anatomy scientific illustrations: child thorax organs - thymus stock illustrations. For Thymus hyemalis, TNF-α secretion was lower than the nonactivated controls for fraction concentrations of 25 μg/mL. After incubation, samples were centrifuged at 12000 g, 4°C for 10 minutes. Thyme extracts significantly reduced production and gene expression of the proinflammatory mediators TNF-α, IL-1B, and IL-6 and highly increased these parameters on the anti-inflammatory IL-10 cytokine. Le genre Thymus comprend de nombreuses espèces, environ 300. Mixtures of n-heptane, ethyl acetate, methanol, and water (5:2:5:2 and 4:1:4:1 v/v), allowed purification of eugenol, 1-octen-3- ol, borneol, thymol, … Trouvé à l'intérieur â Page 748127 133 : P 168160r thyme Thymus vulgaris , Thymus vulgaris ; evaluation of solar dried thyme herbs ... liq . disinfecting compn . for hard surfaces contg . , 131 : P 262696a Thymus vulgaris , Thymus vulgaris ; method and app . for ... Management strategies using natural products, e.g., plant extracts, might be alternatives for control of this pest. The column used was a ZB-5 (Zebron) capillary column, 30 m × 0.32 mm I.D, and 0.25 μm phase thickness. Major bioactive compounds of the extracts from thyme are carvacrol and thymol [12, 13]. Sign up for newsletter today. Comparison with GAPDH as endogenous worked out similar results (data not shown). Trouvé à l'intérieur â Page 398... also Thymus vulgaris ct. thujanol-4 Thujone, 23f, 38 Thyme altitude and light variation, 9 antifungal effects, 63 chemotypes, 7â9, 7t. see also entries beginning Thymus Thymol sedative properties, 82t therapeutic effects, 76 Thymus ... exhibited a total number of 171 amplicons for all primer combinations with an average of 42 bands. Main compounds present were thymol, 1,8-cineole, carvacrol, and borneol. 2000;51:175–9. IL-1β concentration did not decrease when thyme fraction concentrations were increased. Potentially irritating. Compounds thymol, carvacrol, borneol, and linalool were identified by comparison with standard mass spectra obtained in the same conditions and compared with the mass spectra from library Wiley 229. 1999;7:27–8. Essential oils of thyme and their components carvacrol and thymol inhibited 3-nitrotyrosine formation, biomarker of the oxidative stress, supporting the nutraceutical value of thyme and the potential of thymol and carvacrol in preventing the formation of toxic products by the action of reactive nitrogen species [36]. Thymol, thyme, and other plant sources: Health and potential uses. There have been demonstrated effects on the treatment of colitic mice with essential oils of thyme and oregano containing thymol and carvacrol as their principal bioactive compounds, decreasing levels of the proinflammatory cytokines IL-1β, IL-6, and TNF-α. A pilot study was conducted to establish the dose of S. aureus (KY698020 . Natural Antibiotic Oregano in Hydroxyapatite-Coated Titanium Reduces Osteoclastic Bone Resorption for Orthopedic and Dental Applications. Thymus vulgaris L. (Lamiaceae) is a well-known medicinal plant that contains important compounds such as thymol and carvacrol and it has been used in many pharmaceutical dosage forms. Studied plant parts: leaves, flowers and mixed (all plant). The essential oils obtained from Thymus vulgaris L. harvested at four ontogenetic stages were evaluated for their biological activity and chemical composition. Thymus hirsutus, Thym hirsute Extracts from the three thyme species were fractionated using a two-cascade depressurized system and samples were collected in each of the two (separators 1 and 2) separators. The aqueous (upper and colorless) phase was transferred to a new tube. Cytokines are considered to be key players in the inflammatory response involved in atherosclerosis and other Chronic Inflammatory-Based Diseases (CIBDs). It has a spicy flavor when added to your recipes. Irradiation is a feasible and safe decontamination technique, being applied to several types of foods including edible and medicinal plants. Please enable it to take advantage of the complete set of features! At 48 hours, gene expression in cells treated with Thymus zygis S1 and S2 and Thymus vulgaris S1 extracts increased significantly, but Thymus vulgaris S2 and Thymus hyemalis did not change expression of IL-10 compared to oxLDL-activated cells. Prevention and treatment information (HHS). After differentiation, the cells were washed with PBS and treated with or without Cu2+ oxidized LDLs to activated or not activated them. 2021 Apr 7;26(8):2124. doi: 10.3390/molecules26082124. Download the technical datasheet TDS - ENGLISH. THP-1 cells were differentiated in 24-well plates. RQ values represented in Figures 5 and 6 are equivalent to mRNA expression. Trouvé à l'intérieur â Page 180Matrix Analytes Experimental remarks Dried thyme (Thymus vulgaris) Sunflower oil Aromas (thymol and carvacrol) The ultrasound technique (1:10, 98.3W, 50.18°C, 22min) using sunflower oil as solvent and thyme, provide highest recovery of ... Therefore, there is an increasing interest in finding alternative treatments with fewer side effects. The imbalanced expression of cytokines has been implicated in the progression of many diseases including CIBDs [31]. The development of acute bronchitis is often preceded by symptoms of upper . For example, it has been reported that thyme oil reduces NO production in J774A.1 murine macrophages [11]. Fiches des plantes du même genre. Greek Thymos, “spiritedness, expressed passion”, is where the name thyme comes from. Anti-Inflammatory Property. As for Thymus hyemalis, the increase in IL-10 secretion was lower and only significant when the fractions were 15 μg/mL or higher concentrations. 2.2. Despite the large reduction of TNF-α secretion at 24 hours treatments, treatment with fractions of Thymus zygis and Thymus vulgaris for 48 hours showed a lesser reduction of TNF-α. The effect of Thymus vulgaris essential oil (TEO) and its isolated constituents thymol and cavacrol (CVL) were studied in the following experimental models: ear edema, carrageenan-induced pleurisy .
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